Dev115048 1..8
نویسندگان
چکیده
The capability to conditionally inactivate gene function is essential for understanding themolecular basis of development. In geneandmRNA targeting approaches, protein products can perdure, complicating genetic analysis. Current methods for selective protein degradation require drug treatment or take hours for protein removal, limiting their utility in studying rapid developmental processes in vivo. Here, we repurpose an endogenous protein degradation system to rapidly remove targeted C. elegans proteins. We show that upon expression of the E3 ubiquitin ligase substrate-recognition subunit ZIF-1, proteins tagged with the ZF1 zinc-finger domain can be quickly degraded in all somatic cell types examined with temporal and spatial control. We demonstrate that genes can beengineered to becomeconditional lossof-function alleles by introducing sequences encoding the ZF1 tag into endogenous loci. Finally, we use ZF1 tagging to establish the site of cdc-42gene function duringacell invasion event. ZF1 tagging provides a powerful new tool for the analysis of dynamic developmental events.
منابع مشابه
Dev115048 4640..4647
The capability to conditionally inactivate gene function is essential for understanding themolecular basis of development. In geneandmRNA targeting approaches, protein products can perdure, complicating genetic analysis. Current methods for selective protein degradation require drug treatment or take hours for protein removal, limiting their utility in studying rapid developmental processes in ...
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تاریخ انتشار 2014